How to Do Tissue Culture at Home
Welcome to the best hobby ever: micropropagationStart doing TC today with the Plants in Jars Starter Kit 🌱🧪👩🔬 https://bit.ly/4qdw4QB
Shop my Lab on Amazon: https://www.amazon.com/shop/plantsinjars_tc
https://discord.gg/p6yp2Ckv5g
https://www.instagram.com/plantsinjars_tc/
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SUPPLIES LIST
Magnetic stirrer https://amzn.to/459tOS6
1-liter glass beaker https://amzn.to/4s39vQn
Digital scale (0.01 g precision recommended) https://amzn.to/4s1fANg
Pour/weigh boats https://amzn.to/499D37f
Micropipette (200 - 1000 uL is the size you need) https://amzn.to/4oOEWLf
Pipette tips (compatible with your pipette) https://amzn.to/3My9js4
pH probe ("For General Water" model) https://amzn.to/3MGvvA8
pH up & down solutions https://amzn.to/4afnYSF
Pressure cooker https://amzn.to/3KT9ZHP
Pressure cooker racks https://amzn.to/4pFyvLV
Instant Pot (if you don't want to use a pressure cooker) https://amzn.to/4pciE6B
Plants in Jars Starter Kit https://bit.ly/4qdw4QB
Polypropylene (PP) Containers https://amzn.to/3XVAu2p
PP Lids https://amzn.to/4q7mw9w
Forceps https://amzn.to/4rVSFmj
Scissors https://amzn.to/4rWjJSq
Tween 20 https://amzn.to/48TThQQ
Culture Racks https://amzn.to/4oOSJ4F
LED lights https://amzn.to/49fEEaL
Outlet timer https://amzn.to/4q7PK8m
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00:00 Meet our willing victim (African Violet)
00:55 Kitchen Counter Science
01:36 Protocols
02:43 Making the Forbidden Jello
04:41 Cosplaying breaking bad in my kitchen
05:46 Plant Hormones
06:40 Explaining how to use a pipette
07:28 dealing with pH
08:16 A recent change to my method (updated lore)
08:38 The Benefits of PP and why you should use it
09:14 Pouring the Media
09:52 Pressure Cooking the Media
10:36 Rapid fire Q&A
11:00 Unloading the pressure cooker
11:14 other supplies (you'll see what we need these for later)
11:52 Introducing the Still Air Box
12:46 Collecting the tissue sample
13:28 Washing the leaves
14:08 ADDRESSING MY HATERS
14:33 Bleaching the plants (perfectly ethical btw)
15:17 Rinsing the Explants
15:50 Sterile Transfers
18:00 Tissue Culture HACK
19:04 Lighting & Temperature
19:29 Troubleshooting Common Issues
20:46 Subculturing
21:38 bye
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Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
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